Flow chamber system FCS1c
The bottom of the flow channel is formed by the corpus, the side wall – by the gasket, and its top – by the cover slip. The cover slip may be pre-coated with a cell monolayer in a multi-well plate, and will be fixated on the flow chamber by a clip-on spring jacket. This allows an easy exchange of the cultures to be investigated. Modifying the thickness of the gasket and/or the width of its gap, the channel geometry may be altered with the aim to use a whole range of well-defined shear stress. The flow chamber was designed to allow fluorescence microscopic observation of a perifused cell layer at high resolution.
| OFFER NO | PRODUCT |
|---|---|
| 303 0000 | Flow chamber, complete |
| 303 0100 | Main corpus with tubing adapter |
| 303 0510 | Clip-on spring, pack of 10 |
| 303 0521 | Cover slips ø 25 mm, pack of 10, thermanox |
| 303 0522 | Cover slips ø 25 mm, pack of 10, glass |
| 303 0530 | Chamber tubing (complete with Luer fitting), pack of 2 |
| 303 0541 | Silicon gasket (h = 0.3 mm), pack of 10 |
| 303 0543 | Silicon gasket (h = 0.5 mm), pack of 10 |
Specific advantages
- Ability to observe cultures fluorescence microscopically
- Use of coverslips pre-coated with cell cultures
- Easy assembling by means of a clip-on spring jacket
- Generation of defined shear stresses in the flow channel
- Possibility to position the monolayer at the bottom or on top of the media flow
- Variety in flow channel geometry by modifying the gasket
- Leak-proof set-up of the flow chamber
Technical data
Channel length: 15 mm
Channel width : ≥ 2 mm
Channel height: 300 µm
Working distance: 1 mm
Shear stress: 0 ÷ 3 Pa
