Nucleic acid processing

Provitro’s quality management certified lab offers services for total RNA isolation from cells and various tissue types. Total RNA is isolated from your material, using the QIAGEN RNeasyTM system. Purified RNA will be aliquoted as necessary for further investigation according to the customer’s need and will be stored clearly labelled at -80°C. All processes are accomplished according to standard operation procedures (SOP). Quality control and product release are arranged according to clearly defined criteria.

Standard quality control
Standard quality control is run for the purpose of checking on the ratio between absorbance values at 260 and 280 nm. That ratio provides for an estimate of the purity of RNA with regard to contaminants absorbed in the UV, such as proteins. The results allow to quantify the total amount of purified RNA and reveal possible sample contaminations detectable in the UV-range.
Advanced quality control
On request, each sample may be tested regarding RNA degradation or signs of apoptose, using the Agilent 2100 Bioanalyser System. The resulting electropherogramme is checked for baseline development, marker peak and ribosomal signal intensity (ratio 28S/18S RNA).

Nucleic acid processing
OFFER NO	PRODUCT
906 0101	RNA extraction from primary cells, cells not included
906 0102	DNA extraction from primary cells, cells not included
906 0201	RNA extraction from cell lines, cells not included
906 0202	DNA extraction from cell lines, cells not included
906 0311	Provisional gene targeting by RNAi in primary cells
906 0312	Provisional gene targeting by RNAi in cell lines
906 0501	RNA extraction from FFPE* tissue block
906 0502	DNA extraction from FFPE* tissue block
906 0511	RNA extraction from frozen tissue block
906 0512	DNA extraction from frozen tissue block
906 0601	Manual and laser capture microdissection

*FFPE - formalin fixed, paraffin embedded