Skeletal muscle cell growth and differentiation media
Provitro’s skeletal muscle cell growth media were developed to provide skeletal muscle cells with optimal growth conditions in a low serum environment. After confluence, following addition of differentiation medium, the proliferating satellite cells start to form syncytia. Provitro’s skeletal muscle cell basal media are supplemented with FCS 5%, recombinant human EGF and bFGF, fetuin, dexamethasone and insulin for the growth medium and with insulin for the differentiation medium, respectively.
Based on the expertise of provitro and some of our customers, our skeletal muscle cell growth media are recommended for skeletal muscle cells of the species listed below:
Skeletal muscle growth medium, low serum
- Human skeletal muscle cells.
| OFFER NO | PRODUCT | SPECIFICS | DETAILS |
|---|---|---|---|
| 200 0602 | Skeletal muscle cell growth medium, basal | basal |
| SUBSTANCE | FINAL CONC./ML GROWTH MEDIUM |
|---|---|
| FCS or HuS | 000.05 ml |
| Fetuin | 050.00 μg |
| human rec. EGF | 010.00 ng |
| human rec. bFGF | 001.00 ng |
| Insulin | 010.00 μg |
| Dexamethason | 400.00 ng |
| Gentamicin | 050.00 μg |
| Ampho B | 050.00 ng |
| SUBSTANCE | FINAL CONC./ML GROWTH MEDIUM |
|---|---|
| Insulin | 10.0 μg |
| Gentamicin | 50.0 μg |
| Amphothericin B | 50.0 ng |
